The Kotton and Alysandratos Labs have an open position for a laboratory technician. Our labs are part of the Center for Regenerative Medicine (CReM) at Boston University. You will work as part of a highly motivated team focused on studying interstitial lung disease using iPSC-derived lung lineages. We offer exciting research and a strong focus on mentorship. You will learn a wide variety of techniques, such as pluripotent stem cell culture, directed differentiation, RT- qPCR, flow cytometry, immunofluorescence and imaging.
Responsibilities: You will be working in the lab, performing experiments, analyzing data and reporting results.
Specific duties will involve:
- Cell culture: human iPSC maintenance, expansion and routine mycoplasma testing
- Directed differentiation of human iPSCs into lung epithelial and lung mesenchymal cells, establishment of epithelial-mesenchymal co-cultures
- Flow cytometric assessment of cells
- Single cell RNA sequencing: sample preparation, optimizing workflow, assist in the analysis of omics data
- RNA extraction and RT-qPCR
- Protein analysis, such as Western blot and immunofluorescence (IF)
- Experimental planning, recording of results, data analysis, and presentation
- Help maintaining an efficiently operating lab (ordering, keeping logs and inventories up to date)
- Actively contributing to a welcoming and supportive lab environment, including mentoring junior team members
Skills
Required qualifications: A Bachelor’s degree in Biology or a related field is required. Excellent organizational and communication skills and ability to work as part of a team. Candidates with prior experience working in a laboratory environment are encouraged to apply. An excellent candidate will have experience with cell culture and/or standard molecular biology techniques (RNA extraction, RT-qPCR, immunofluorescence)
We are an equal opportunity employer, and all qualified applicants will receive consideration for employment without regard to race, color, natural or protective hairstyle, religion, sex, age, national origin, physical or mental disability, sexual orientation, gender identity, genetic information, military service, pregnancy or pregnancy-related condition, or because of marital, parental, or veteran status. We are a VEVRAA Federal Contractor.
